Preparation:

1. Start a new experiment on the data collection system

2. Connect the colorimeter to the data collection system using the extension cable

3. Calibrate the colorimeter

4. Put about 20 mL of the crystal violet solution, the sodium hydroxide solution, and the distilled water in separate 50-mL beakers. Label the beakers.

5. Cover the NaOH solution with a watch glass

6. Label one syringe "CV", one "NaOH:, and one "Water"

7. For the first run, fill the three syringes with the volume of liquids specified in Table 5, Trial 1.

8. Eliminate the air bubbles from the syringes by holding the syringe over a sink, pointing upward, knocking gently on the syringe and pushing the plunger in until all the air is expelled. Draw in more solution as needed.