DRAFT: This module has unpublished changes.

Equipment:

- C18 Sep-Pak cartridge

- Syringe, 1-mL

- Syringe, 10-mL, or dropper bottle or wash bottle

- Graduated cylinder, 10-mL

- 18% Isopropanol, 100 mL

- Unsweetened Kool-Aid drink, 10 mL

- Distilled water


Method:

Perform the following steps three times and record your data for each trial


1. Use a 10-mL syringe to flush the C18 Sep-Pack cartridge with 10 mL of undiluted 10% isopropanonl and record the rate at which it is done


2. Collect the eluate in a 10-mL graduated cylinder to determine the flow rate


3. Using the 10-mL syringe, flush the Sep-Pack cartridge with 10 mL of distilled water


4. Slowly add the Kool-Aid into the Sep-Pack cartridge


5. Collect the effluent tat washed out of the column as the Kool-Aid is added


6. Fill the 10-mL syringe with 18% isopropanol solution and pump it through the Sep-Pack cartridge at a steady rate

HINT: add weights to the top of the 10-mL syringe to help keep the slow, steady rate


7. Collect the eluent in the 10-mL graduated cylinder


8. Record hte volume of the liquid collected in the graduated cylinder when the red or blue dye is first observed in the eluent from the Sep-Pack colums (Vr(start)) and when that color is no longer observed (Vr(end))

HINT: there may be an area of overlap that will be purple


9. Clean up according to your teacher's instructions

DRAFT: This module has unpublished changes.