Equipment:
- C18 Sep-Pak cartridge
- Syringe, 1-mL
- Syringe, 10-mL, or dropper bottle or wash bottle
- Graduated cylinder, 10-mL
- 18% Isopropanol, 100 mL
- Unsweetened Kool-Aid drink, 10 mL
- Distilled water
Method:
Perform the following steps three times and record your data for each trial
1. Use a 10-mL syringe to flush the C18 Sep-Pack cartridge with 10 mL of undiluted 10% isopropanonl and record the rate at which it is done
2. Collect the eluate in a 10-mL graduated cylinder to determine the flow rate
3. Using the 10-mL syringe, flush the Sep-Pack cartridge with 10 mL of distilled water
4. Slowly add the Kool-Aid into the Sep-Pack cartridge
5. Collect the effluent tat washed out of the column as the Kool-Aid is added
6. Fill the 10-mL syringe with 18% isopropanol solution and pump it through the Sep-Pack cartridge at a steady rate
HINT: add weights to the top of the 10-mL syringe to help keep the slow, steady rate
7. Collect the eluent in the 10-mL graduated cylinder
8. Record hte volume of the liquid collected in the graduated cylinder when the red or blue dye is first observed in the eluent from the Sep-Pack colums (Vr(start)) and when that color is no longer observed (Vr(end))
HINT: there may be an area of overlap that will be purple
9. Clean up according to your teacher's instructions