Discussion/Conclusion:

  The purpose of this lab was to separate the blue and red components that make Kool-Aid using chromatography. This was done using a C18 Sep-Pack chromatography column. The cartridge was flushed with 18% isopropanol, distilled water, and certain dye samples. The least polar component is retained in the cartridge and comes out last while the most polar component come out first. This technique is how the components were separated. After several calculations, the longer cartridge was more efficient. Based on the results obtained from the experiment, the components of the mixture of Kool-Aid were separated well. Some sources for error are basic human error, like incorrect measurements, and flushing the cartridge at a rate that was too rapid. This experiment could be made better in the future by using a more polar solvent to make sure the separation is the most polar substances first and the least polar substances last.