DRAFT: This module has unpublished changes.

Equipment:

  • Sep-Pack, (single)
  • Sep-Pack, (triple)
  • Syringe, 1-mL
  • Syringe, 10-mL
  • Graduated Cylinder, 10-ml
  • 18% Isopropanol, 100 mL
  • 800g weight
  • Unsweetened Kool-aid® drink, 10 mL
  • Deionized Water
  • Stand, with vise

 

Method:

  • Gathered Equipment, assembled stand with vise, prepared unsweetened Kool-aid® drink according to directions from TA.
  • Used 10-mL syringe to flush Sep-Pack with 10 mL 18% Isopropanol. Used vise and stand to stabilize syringe and Sep-Pack, 800g weight to ensure steady rate of flow, and graduated cylinder to collect the eluate and monitor the rate of flow.
  • Disposed of eluate in graduated cylinder. Cleaned cylinder.
  • Flushed Sep-Pack with 10 mL of deionized water.
  • Injected 1 mL sample of unsweetened Kool-aid® drink into Sep-Pack, using 1-mL syringe. 
  • Collected and discarded effluent that washed out while injecting the sample.
  • Filled 10 mL syringe with 18% Isopropanol, attached to sep pack, and mounted on stand using vise. Placed cleaned graduated cylinder beneath, to collect eluent.
  • Using weight to ensure even flow, pumped solvent through Sep-Pack, collecting eluent in the graduated cylinder below. 
  • Measured the shifts in dye color, as seen in table 2, recording how much eluent flowed through for each to begin, then end.
  • Repeated test with triple Sep-Pack.
  • Disposed of eluent according to directions from TA, cleaned all containers and syringes, and returned all equipment to its point of origin.
DRAFT: This module has unpublished changes.