DRAFT: This module has unpublished changes.


  • Data Collection System
  • Spectrophotometer (Red Tide)
  • Sensor Extension Cable
  • Cuvette
  • Beaker (3), 50-mL
  • Syringe (3), 1-mL
  • Plastic Covers
  • 0.1 M Sodium Hydroxide, 20 mL
  • 1.2e-5 M Crystal Violet, 20 mL
  • Water, Deionzed, 30 mL
  • Marking Pen
  • Kimwipes


  • Gathered all equipment and materials. Inspected all equipment for damage or contamination.
  • Set up Data Collection System.
  • Calibrated the Spectrophotometer.
  • Put 20 mL of Crystal Violet solution in beaker. Repeated with the distilled water, and the Sodium Hydroxide. Covered Sodium Hydroxide with the plastic covering.
  • Labeled each syringe after each solution. Filled according to Table 1, taking care to remove any air bubbles.
  • Table 1: Initial concentrations and Volumes
  • Inserted specified quantity of water and NaOH into clean cuvette, before beginning the data recording process, injecting the Crystal Violet solution, and inverting it rapidly.
  • Immediately wiped the cuvette with a Kimwipe, then inserted it into the Red Tide Spectrophotometer.
  • Recorded data until absorbance had decreased below 0.1.
  • Saved data, then repeated process two more times, following the concentrations and volumes from Table 1 for trials 2 and 3 respectively.
  • Disposed of solutions in designated containers. Cleaned all glassware and cuvettes, set them to dry in their designated areas, and returned all equipment to its point of origin.
DRAFT: This module has unpublished changes.