Equipment:

• Data Collection System
• Spectrophotometer (Red Tide)
• Sensor Extension Cable
• Cuvette
• Beaker (3), 50-mL
• Syringe (3), 1-mL
• Plastic Covers
• 0.1 M Sodium Hydroxide, 20 mL
• 1.2e-5 M Crystal Violet, 20 mL
• Water, Deionzed, 30 mL
• Marking Pen
• Kimwipes

Methods:

• Gathered all equipment and materials. Inspected all equipment for damage or contamination.
• Set up Data Collection System.
• Calibrated the Spectrophotometer.
• Put 20 mL of Crystal Violet solution in beaker. Repeated with the distilled water, and the Sodium Hydroxide. Covered Sodium Hydroxide with the plastic covering.
• Labeled each syringe after each solution. Filled according to Table 1, taking care to remove any air bubbles.
• Table 1: Initial concentrations and Volumes
• Inserted specified quantity of water and NaOH into clean cuvette, before beginning the data recording process, injecting the Crystal Violet solution, and inverting it rapidly.
• Immediately wiped the cuvette with a Kimwipe, then inserted it into the Red Tide Spectrophotometer.
• Recorded data until absorbance had decreased below 0.1.
• Saved data, then repeated process two more times, following the concentrations and volumes from Table 1 for trials 2 and 3 respectively.
• Disposed of solutions in designated containers. Cleaned all glassware and cuvettes, set them to dry in their designated areas, and returned all equipment to its point of origin.