Calculations

 

Calculating the band width for the two components

Trial 1: Wred

1.1-0.2=0.9

Wblue

2.3-0.3=2.0

 

Trial 2: Wred

1.2-0.1=1.1

Wblue

2.0-0.3=1.7

 

Calculating the center of both bands optained

Trial 1: Vr(avg,red)=0.2mL + (0.5)(0.9mL)=0.65

Vr(avg,blue)=0.3mL + (0.5)(2.0mL)=1.3

 

Trial 2: Vr(avg,red)=0.1mL + (0.5)(1.1mL)=0.65

Vr(avg,red)=0.3mL + (0.5)(1.7mL)=1.15

 

Calculating the mobile phase volume for each trial

 

 

Calculating the retention factor

Trial 1:

 

 

 

Trial 2:

 

 

Calculating the separation

Trial 1:

a=1.65/0.33=5

 

Trial 2:

a=1.35/0.33=4.09

 

Calculating Theoretical plate number

Trial 1:

 

Trial 2:

 

Calculating the Resolution 

Trial 1: 

 

Trial 2: