Week 1: The Set Up

November 7, 2011

Group 6

PURPOSE:

The purpose of this lab is to grow Wisconsin Fast Plants under different light regimes for a week and compare the different phenotypes and protein expressions.

INTRODUCTION

To begin this five week experiment, four Wisconsin Fast Plants with different phenotypes were planted in order for the growth to be observed. In the plant tray, the phenotypes were scattered so that no same phenotype was next to eachother. The phenotypes consisted of Verigated, Purple Stem, Non-Purple, and Standard. Once ready, the plants were placed under a chosen light regime using a light box created by Dr. Folta from University of Florida. The growth of each plant was monitored daily for a week.

METHODS

1. Obtain two of each of the phenotypes of fast plants.

2. Fill each cell 3/4 with soil and randomly disperse the plants in the cells so that none

of the same phenotypes are next to each other.

3. Put the cells in the correctly labeled box with a hole for the light to rest on.

4. Set the light box up for the light regime chosen (FR:9, R: 1, B: 1, G: 1) and turn it on.

5. Measure the stem growth of the plants daily around the same time for a week.

CONCLUSIONS

Each plant was placed randomly in a cell and put under the light box that contained mostly far-red light. The plants were measured for 6 days between 3 and 5 daily.